5.3.1 One Way ANOVA

Summary

Statistics has two modes of data input; indexed and raw. When a statistical analysis is performed, there is usually no need to use an entire data set; Origin provides several methods of data selection. For example, the interactive Regional Data Selector button can be used in the graphical selection of data, or the Column Browser dialog can be employed to make the selection.

In this tutorial, you will learn how to use the two methods of data input so that the Analysis of Variance (ANOVA) statistical test can be performed.

ANOVA is a parametric method for means comparison of several groups, and it is also an extension of two independent sample t-tests. ANOVA is more powerful than multiple t-tests since it controls the chance to commit type I error better when the number of groups is relatively large. For example, when comparing the means among 5 groups using a t-test, ten multiples of the t-test, each with a significance level of 0.05, are performed, resulting in an overall chance to commit type I error of 1-(1-0.05)^10=0.401. Under the same circumstances, the chance to commit type I error in ANOVA is 0.05.

ANOVA requires normality and equal variance. If these requirements are not met, non-parametric analysis should be used.

Minimum Origin Version Required: Origin 2016 SR0

What You Will Learn

This tutorial will show you how to:

  • Use different input data modes in the statistical analysis dialog
  • Do normality tests
  • Perform one-way ANOVA

Steps

Origin can perform ANOVA from an indexed data mode, or from a raw data mode.

When an indexed data mode is used, the data needs to be organized in a factor column and a data column.

Tutorial ANOVA 008.png

When a raw data mode is used, the data needs to be organized with different levels in different columns.

Tutorial ANOVA 009.png


Indexed Data Mode

Nitrogen content, in milligrams, has been recorded for 4 kinds of plants. We are interested to know whether different plants have significantly different nitrogen content. As a result, we will use the index data mode in One-Way ANOVA analysis.

  1. Start with a new workbook. From the Menu Bar, click Data: Import from File: Single ASCII..., browse to the Samples\Statistics folder and double click on nitrogen.txt. In the Import and Export:impASC dialog box, click the OK button.
  2. Next, we perform a normality test on each group of data to determine whether it follows a normal distribution. Highlight the first column of the workbook and from the Menu Bar, select Worksheet: Sort Worksheet: Ascending.
  3. From the Menu Bar, select Statistics: Descriptive Statistics: Normality Test: Open Dialog. In the Normality Test dialog, in the Input tab, expand Input Data and Range 1. Press the triangle button at the right of Data Range and select B(Y): nitrogen, then press the triangle button at the right of Group and select A(X): plant. Click OK.
  4. DOC-2411 ANOVAn1a Magenta.png

  5. With the nitrogen worksheet active, go to the Menu Bar and open Statistics: ANOVA: One-Way ANOVA.... In the ANOVAOneWay dialog box, in the Input tab, set Input Data to Indexed. Pressing the triangle button at the right of Factor, select A(X): plant and pressing the triangle button at the right of Data, select B(Y): nitrogen.
  6. DOC-2411 ANOVA03a Magenta.png
  7. In theMeans Comparison tab, check the Tukey check box; In the Tests for Equal Variance tab, check the Levene check box; In Power Analysis tab, select Actual Power check box; In the Plots tab, check the Means Plot[SE as Error] and the Means Comparison Plot check boxes. Click OK.
DOC-2411 ANOVA03b Magenta.png

Result Interpretations

  • On the ANOVA1Way1 report sheet, you will see a Homogeneity of Variance Test node. If results are not visible, click to expand the node. Because the p-value is greater than 0.05, the four groups are considered to have equal variance.
ANOVA04.png
  • The ANOVA table (Overall ANOVA) reports a p-value that is smaller than 0.05, hence at least two of the four groups have significantly different means.

ANOVA05.png

  • Expanding the results of "Means Comparisons" reveals further information regarding relationships between groups.
ANOVA06.png

  • Here we see that the mean of PLANT4 differs significantly from the other three groups. Looking at the Means Plot and Means Comparison Plot, we can see that PLANT4 has the smallest mean and is significantly different from the other three groups.
ANOVA07.png
ANOVA09.png
  • In the "Powers" table, Actual Power=0.99976, that is, the chance of a Type II error occurring is almost equal to zero.
ANOVA10.png

Raw Data Mode

  1. Start with a new workbook. From the Menu Bar, click Data: Import from File: Single ASCII..., and double click on nitrogen_raw.txt. In the Import and Export:impASC dialog box, click OK .
  2. Highlight all columns, and from the Menu Bar, click Statistics : ANOVA : One-Way ANOVA. In the ANOVAOneWay dialog, in the Input tab, set Input Data to Raw, and click OK.
  3. Repeat the examination of analysis results and you will see that we reach the same conclusions as we did when using Indexed data mode.


DOC-2411 ANOVA311a.png

If the Level Name is set to Auto, it will follow the column long name of the selected data.