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OriginLab Technical Support
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Create volcano plot for fold change and p-value data.

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This tool can be used to create scatter plot of p-value versus fold change for microarray data. The graph is composed of six regions. The plot style for each region can be individually customized.

Download the file VolcanoPlot.opx, and then drag-and-drop onto the Origin workspace. An icon will appear in the Apps Gallery window.


  1. Click the Volcano Plot icon in the Apps Gallery window to open the dialog.
  2. Choose XY data from a worksheet: fold change for X and p-value for Y. If gene names or probe set IDs are available in the worksheet, choose them as Label.
  3. If X data is linear, check Log2 Transform for X check box to convert to log 2 scale.
  4. In Cutoff Values group, specify cutoff values for P-value and Fold Change. P-value must be between 0 and 1. Fold change must be larger than 1. Both of these two cutoff values are linear scale.
  5. Check Show Reference Lines option to add a horizontal line for the cutoff P-value and two vertical lines for the cutoff fold change in the plot.


  1. To customize symbol color or size for each region of the plot, double click on the region to open Plot Details dialog, and change settings in the Symbol tab.
  2. To customize reference line style, right click on the line, then  choose Properties from the context menu. In the Object Properties dialog, change settings in the Line tab.


11/13/2017 v1.1 Fixed treenode bug in Origin 2018.

Reviews and Comments:
02/13/2023OriginLabHi, giorgiocorsi

Could you let us know which parameters you feel are not clear yet?

OriginLab Technical Support

02/10/2023giorgiocorsiWorks fine, but some settings/parameters should be better explained


03/10/2022yukiOriginlabHi ORIGINLABTEST2, could you please tell us what went wrong when you were using this app? We can help to fix the problem.
OriginLab Technical Support

03/10/2022ORIGINLABTEST2does not work

03/07/2022OriginLabHi dhmast_pnnl,
I am afraid we do not reproduce your question in Origin2021. If you do not mind, please send your project to us, then we can reproduce this issue.

03/04/2022dhmast_pnnlNot working in OriginPro 2021. I Press okay, but the plot doesn't load in a new window. nothing happens


10/24/2021OriginLabHi xiasang0506, please make sure that you are using this app on Origin 2016 or later versions. If you still have a problem, please contact us via
OriginLab Technical Support

10/23/2021xiasang0506it does not work! worst!