File Exchange > Graphing >    Paired Comparison Plot

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Paired Com...ot.opx
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Create plots with significant differences for pairwise comparison

Screen Shot and Video:

This app can be used to create various plot with significant differences.  Use can choose what post-hoc method to be used for getting the statistics of means comparison.

Download the file "Paired Comparison Plot.opx", and then drag-and-drop onto the Origin workspace. An icon will appear in the Apps gallery window.


  • Click the app icon to open the dialog
  • Select  input for Data and Group columns. Please note that the you can select one group column for One-way ANOVA, or two group columns for Two-way ANOVA.
  • You can choose to indicate the significant difference on top with letters, asterisk bracket, or p values.


  • To view the sample graphs, right click on the app icon and choose Open Samples Folder from the context menu
  • Significant Mark option
    • Letters on Top
    • Asterisk with Bracket
    • P-value with Bracket
  • Plot Type option
    • Bar
    • Data Points
    • Box Chart
    • Scatter with Error Bar
  • Means Comparison option
    • Tukey
    • Bonferroni
    • Dunn-Sidak
    • Fisher's LSD
    • Scheffé
    • Holm-Bonferroni
    • Holm-Sidak 


3.6 Fix no label issue for Asterisk with Bracket method in 3.5. Update build-in Sample OPJ
3.5 Options of Error bar to be SD or SE and fix speed problem
3.4 Fix bug of numeric separator issue in GOS
3.3 Fix bug of letters on top option introduced in version 3.1
3.1 Fix problem of large group number and axis label
3.0 Support Raw/Unstack data for one way anova
2.1: Improve Letters on Top option to make letter ordered by mean values
2.0: Support options of mean comparison methods, different plot type
1.01: Fix bug of asterisks displaying problem when p = 0 or p = 0.01

Reviews and Comments:


03/13/2023OriginLabHi g.pugliese,

Have you confirmed that the settings are consistent between the two tools? Or you can share your project file with us so that we can investigate the issue further. Thanks.


03/13/2023g.puglieseI compared significant differences between paired comparison plot and a normal 2 way anova using the same post-hoc test. Sometimes the paired comparison plot fail, i.e it reports n.s. when actually there is a difference. Could you please help?

03/05/2023JonphoonIssue resolved, it was due to differences in my plot settings. Thanks to the Origin team!

02/28/2023OriginLabHi Jonphoon,

Would it be possible for you to share your project file with us so that we can investigate the discrepancy?

02/27/2023JonphoonConveniently plots charts with letters for statistical letters. But sometimes the letters on the chart differs from the means comparison interaction grouping letters table from Anova, although using the same post hoc tests



01/21/2023ZIYACVery good software