File Exchange > Graphing >    Paired Comparison Plot

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Paired Com...ot.opx
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Create plots with significant differences for pairwise comparison

Screen Shot and Video:

This app can be used to create various plot with significant differences.  Use can choose what post-hoc method to be used for getting the statistics of means comparison.

Download the file "Paired Comparison Plot.opx", and then drag-and-drop onto the Origin workspace. An icon will appear in the Apps gallery window.


  • Click the app icon to open the dialog
  • Select  input for Data and Group columns. Please note that the you can select one group column for One-way ANOVA, or two group columns for Two-way ANOVA.
  • You can choose to indicate the significant difference on top with letters, asterisk bracket, or p values.


  • To view the sample graphs, right click on the app icon and choose Open Samples Folder from the context menu
  • Significant Mark option
    • Letters on Top
    • Asterisk with Bracket
    • P-value with Bracket
  • Plot Type option
    • Bar
    • Data Points
    • Box Chart
    • Scatter with Error Bar
  • Means Comparison option
    • Tukey
    • Bonferroni
    • Dunn-Sidak
    • Fisher's LSD
    • Scheffé
    • Holm-Bonferroni
    • Holm-Sidak 


3.6 Fix no label issue for Asterisk with Bracket method in 3.5. Update build-in Sample OPJ
3.5 Options of Error bar to be SD or SE and fix speed problem
3.4 Fix bug of numeric separator issue in GOS
3.3 Fix bug of letters on top option introduced in version 3.1
3.1 Fix problem of large group number and axis label
3.0 Support Raw/Unstack data for one way anova
2.1: Improve Letters on Top option to make letter ordered by mean values
2.0: Support options of mean comparison methods, different plot type
1.01: Fix bug of asterisks displaying problem when p = 0 or p = 0.01

Reviews and Comments:


10/08/2022OriginLabHi 3617,

这个app 生成的图显示的显著性标注,就是对组内变量进行事后比较的结果。
如果需要详细结果报告,请在 Origin 中使用单因素或双因素方差分析(菜单:统计 > ANOVA)



The significance label is not in reverse alphabetical order. It is ordered by mean values, and then comparison. So we can not update the design as you hope. You just can modify the labels manually. For the Box plot, you can edit the labels in the Text object directly. For the Column plot, the label is from the column datasets. You need to go to the result sheet, and then change the label text in the column.

09/08/2022kathy.dibley@csiro.auI have been using the Paired comparison plot v3.6 and have found it very useful, except for one problem. When I run the analysis, the significance level letters are in reverse alphabetical order, so that when I plot them the letters run in reverse from left to right (so starting from d in this instance below). This is despite having the table set up in the same order as visualised on the graph. Is there some way I can fix this?

09/05/2022Zijing Xie 

07/18/2022YU Dehuigood

06/20/2022snowliHi @lp cen,
Could you send dat and clarify the issue so we can check what is not accurate?

Thanks, Snow

06/20/2022lp cen计算不准确